This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target ...
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This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.
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Add this copy of CRISPR Guide RNA Design: Methods and Protocols to cart. $149.66, new condition, Sold by Ria Christie Books rated 4.0 out of 5 stars, ships from Uxbridge, MIDDLESEX, UNITED KINGDOM, published 2021 by Springer-Verlag New York Inc..
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New. Contains: Illustrations, black & white, Illustrations, color. Methods in Molecular Biology . X, 286 p. 56 illus., 46 illus. in color. Intended for professional and scholarly audience.
Add this copy of Crispr Guide RNA Design: Methods and Protocols to cart. $244.90, new condition, Sold by Ria Christie Books rated 4.0 out of 5 stars, ships from Uxbridge, MIDDLESEX, UNITED KINGDOM, published 2020 by Humana.
Add this copy of CRISPR Guide RNA Design: Methods and Protocols to cart. $140.50, new condition, Sold by Ingram Customer Returns Center rated 5.0 out of 5 stars, ships from NV, USA, published 2021 by Springer-Verlag New York Inc..
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Seller's Description:
New. Print on demand Contains: Illustrations, black & white, Illustrations, color. Methods in Molecular Biology . X, 286 p. 56 illus., 46 illus. in color. Intended for professional and scholarly audience.
Add this copy of Crispr Guide RNA Design: Methods and Protocols to cart. $243.84, new condition, Sold by Ingram Customer Returns Center rated 5.0 out of 5 stars, ships from NV, USA, published 2020 by Humana.
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New. Print on demand Sewn binding. Cloth over boards. 286 p. Contains: Unspecified, Illustrations, black & white, Illustrations, color. Methods in Molecular Biology, 2162.
Add this copy of Crispr Guide Rna Design: Methods and Protocols (Methods to cart. $170.59, good condition, Sold by Bonita rated 4.0 out of 5 stars, ships from Santa Clarita, CA, UNITED STATES, published 2021 by Humana.
Add this copy of Crispr Guide Rna Design: Methods and Protocols (Methods to cart. $219.19, new condition, Sold by Bonita rated 4.0 out of 5 stars, ships from Santa Clarita, CA, UNITED STATES, published 2021 by Humana.
Add this copy of Crispr Guide Rna Design: Methods and Protocols (Methods to cart. $304.30, new condition, Sold by Just one more Chapter rated 3.0 out of 5 stars, ships from Miramar, FL, UNITED STATES, published 2020 by Humana.
