This text provides in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results. In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in-situ hybridization, are documented thoroughly. Preceding the first ...
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This text provides in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results. In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in-situ hybridization, are documented thoroughly. Preceding the first chapter are ten single-page Quick Start protocols. Patterned after computer software manuals, these abbreviated instructions provide the experienced student with the fundamental steps to complete the ten microtechnique protocols most frequently used today. Appendices on laboratory practice (chemical toxicities, common calculations and buffer tables) are provided.
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